ELISpot Flex: Cat IFN-γ (ALP)

3122-2A

Publications: 2

Documents

Questions?

Our most adaptable ELISpot format, made to fit into your unique plan. Combine it with a substrate, plate, and/or antigen of choice.

Components

Capture mAb	MT131
Detection mAb	MT114, biotin
Enzyme conjugate	Streptavidin-ALP

In stock

Delivery 4-9 business days

Shipping $0

Complementary products

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ELISpot substrate: BCIP/NBT-plus for ALP

Product specifications

Intended use

This ELISpot Flex kit is intended for the enumeration of cells secreting cat IFN-γ using the ELISpot assay. For research use only. Not for use in diagnostic procedures.

Serum/Plasma samples

Recommendation

Product details

Product	ELISpot Flex: Cat IFN-γ (ALP)
Application	ELISpot
Analyte	IFN-γ
Reactivity	Cat, Lion, Cheetah, Leopard
Specificity	This kit is based on a matched pair of mAbs specific for cat IFN-γ.
Cross-reactivity	Cross-reacts with IFN-γ from lion, cheetah, and leopard.

Shipping and Storage

Shipping	Shipped at ambient temperature.
Storage	Store at 4-8 °C upon receipt.
Shelf life	At least 18 months from date of receipt.

Performance

Cat PBMC (50,000 cells/well) were incubated for 20 h in the presence or absence of PHA, and the number of cells secreting IFN-γ was analyzed by ELISpot.

Documents

Datasheet/Protocol
SDS

Tutorials

Tutorial, Published December 28, 2023

Incubation strategies for success in ELISpot and FluoroSpot

We've made a handy table summarizing incubation times for different analytes to make your research planning for the ELISpot and FluoroSpot assay a little easier.

Tutorial, Published November 24, 2022

Cell stimulation in ELISpot and FluoroSpot

In this feature, we’ll walk you through the optimal use of different polyclonal and antigen-specific stimuli.

Tutorial, Published November 7, 2022

7 common ELISpot errors and how to avoid them

ELISpot troubleshooting. We discuss 7 of the most common issues we’ve encountered in ELISpot, what mistakes causes them and how to avoid and correct them.

Tutorial, Published October 14, 2022

What is a positive ELISpot response?

DFR is a non-parametric statistical test for determining positive responses in ELISpot and FluoroSpot.

Publications (2)

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Analyte information

IFN-γ

Analyte description	Interferon-γ (IFN-γ) is the only type II interferon. This proinflammatory cytokine is secreted by activated T cells and NK cells. It activates macrophages and endothelial cells and regulates immune responses by affecting APCs, T cells, and B cells. Production of IFN-γ by helper T cells and cytotoxic T cells is a hallmark of the Th1-type phenotype. Thus, high-level production of IFN-γ is typically associated with effective host defense against intracellular pathogens.
Alternative names	Interferon-γ, Interferon-gamma, IFN-γ, IFN-gamma, IFN-g, IFNg
Cell type	T cell, Tc, Th1, NK cell
Gene ID	493965